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KMID : 0359319900300010107
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Korean Journal of Veterinary Research
1990 Volume.30 No. 1 p.107 ~ p.112
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Indirect calculation for volume of packed red cell ( VPRC ) by means of erythrocyte sedimentation rate ( ESR ) of blood diluted with isotonic dextrose solution in cattle
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Abstract
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The purpose of this study is to derive indirect values of volume of packed red cell(VPRC), without centrifugation, from erythrocyte sedimentation rate(ESR) of blood diluted with isotonic dextrose solution in cattle.
Each of ten blood samples taken from apprently healthy Korean cows and Holstein cows was used to produce eight different mixture of autologous plasma and blood corpuscles such that their values of VPRC lay between 15 to 50㎖/100㎖, and then each of the blood mixture was again diluted with 5% dextrose solution.
The measurements of ESR using 45 degree-angled capillary hematocrit tube, 1.1∼1.2㎜ bore, (45˚micro-ESR) were practised for the diluted blood of various levels of VPRC under the ambient temperature of 10˚ 20˚and 30℃.
Reliable values of VPRC on the basis of the correlating linear regressive equation to the ESR could be derived from the values of 45˚micro-ESR/hr in the mixture of one part of whole blood and one part of 5% dextrose solution(1:1).
For an aid of practical use, authors suggested a list of the 45˚micro-ESR/hr values of the diluted blood equivalent to VPRC of whole blood.
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